ncRNA basic information
ncRNA ID:
MI0000077
ncRNA Database:
miRBase
ncRNA Name:
miR-21
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR,Northern blot
ncRNA Target Gene:
PTEN
ncRNA Pathway:
PI3K/Akt pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00694 (APRD00521)
Drug Name:
Daunorubicin
Drug Method:
The expression levels of miRNA were determined by Real-time PCR and Northern blot .Those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
leukemia
Tissue/Cell:
cell line (K562,K562/DNR,HEK293T)
Other information
Title:
Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line.
Journal:
FEBS Lett
Published:
2011
PubMed ID:
21187093