ncRNA basic information
ncRNA ID:
MIMAT0004774
ncRNA Database:
miRBase
ncRNA Name:
miR-501-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
MYCN
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In the present study, it was revealed that miR-501-3p expression was decreased in glioma tissues and further underexpressed in cisplatin-resistant glioma cells compared with wild-type (WT) glioma cells. Furthermore, cisplatin treatment inhibited the level of miR-501-3p in a time-dependent way. Ectopic expression of miR-501-3p suppressed glioma cell growth and invasion, but increased cisplatin-resistant glioma cell apoptosis. Furthermore, miR-501-3p sensitized glioma cells to cisplatin-induced proliferation arrest and death. Mechanistically, it was demonstrated that miR-501-3p targeted MYCN in glioma cells. In addition, it was revealed that miR-501-3p inhibited MYCN expression by a luciferase reporter assay and reverse transcription-quantitative polymerase chain reaction. Notably, restoration of MYCN reversed the effects of miR-501-3p in cisplatin-resistant glioma cells. In conclusion, these results suggested that miR-501-3p may serve a promising marker for cisplatin resistance.
Drug Response:
sensitive
Cancer basic information
Cancer:
glioma
Tissue/Cell:
cell line
Other information
Title:
miR-501-3p sensitizes glioma cells to cisplatin by targeting MYCN.
Journal:
Mol Med Rep
Published:
2018
PubMed ID:
30221699