ncRNA basic information
ncRNA ID:
MI0000490
ncRNA Database:
miRBase
ncRNA Name:
miR-206
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
MAPK3
ncRNA Pathway:
MAPK2 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Dual-Luciferase reporter gene assay confirmed targeted regulation between miR-206 and MAPK3. DDP resistant cell line BGC823/DDP and SGC7901/DDP were generated for comparing miR-206 and MAPK3 expression against parental cells using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, followed by flow cytometry measuring apoptosis. Drug-resistant cells were transfected with miR-206 mimic for measuring MAPK3 and phosphorylated MAPK3 (p-MAPK3) expression. Flow cytometry and EdU were employed for measuring cell apoptosis and proliferation.
Drug Response:
resistant
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (BGC823/DDP and SGC7901/DDP)
Other information
Title:
MicroRNA-206 facilitates gastric cancer cell apoptosis and suppresses cisplatin resistance by targeting MAPK2 signaling pathway.
Journal:
Eur Rev Med Pharmacol Sci
Published:
2019
PubMed ID:
30657558