ncRNA basic information
ncRNA ID:
MI0000060/MI0000061/MI0000062
ncRNA Database:
miRBase
ncRNA Name:
Let-7a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
squamous carcinoma
Tissue/Cell:
cell line (A431,HepG2, A10A,MCF-7)
Other information
Title:
Let-7a microRNA suppresses therapeutics-induced cancer cell death by targeting caspase-3.
Journal:
Apoptosis
Published:
2008
PubMed ID:
18758960