ncRNA basic information
ncRNA ID:
MI0000542
ncRNA Database:
miRBase
ncRNA Name:
miR-320a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
MCL1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Fuantitative real-time polymerase chain reaction (qRT-PCR) and those of protein were by Western blot analysis.Fuciferase activity assay was used to verify the target genes of miRNAs.The CCK-8 assay was used to monitor the growth of the cells .Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
resistant
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell lines (MG-63, U2OS, HOS,SAOS-2, hFOB)
Other information
Title:
Long noncoding RNA SNHG12 mediates doxorubicin resistance of osteosarcoma via miR-320a/MCL1 axis.
Journal:
Biomed Pharmacother
Published:
2018
PubMed ID:
30119255