ncRNA basic information
ncRNA ID:
MIMAT0000242
ncRNA Database:
miRBase
ncRNA Name:
miR-129-5p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Wnt5a
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00441 (APRD00201)
Drug Name:
Gemcitabine
Drug Method:
The IC50 for gemcitabine in 20 bladder cancer cells was first profiled from Genomics of Drug Sensitivity in Cancer. miR-129-5p level and gene mRNA expression were detected using quantitative real-time PCR (qRT-PCR). Cell viability, apoptosis, and gene protein level were assessed by MTT, flow cytometry, and Western blot, respectively. Regulatory relationship between Wnt5a and miR-129-5p was determined using luciferase reporter assay.
Drug Response:
resistant
Cancer basic information
Cancer:
bladder cancer
Tissue/Cell:
cell line (UM-UC-3 and SW780)
Other information
Title:
miR-129-5p inhibits gemcitabine resistance and promotes cell apoptosis of bladder cancer cells by targeting Wnt5a.
Journal:
Int Urol Nephrol
Published:
2018
PubMed ID:
30117016