ncRNA basic information
ncRNA ID:
MIMAT0003885
ncRNA Database:
miRBase
ncRNA Name:
miR-454-3p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
STAT3
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Cell migration was detected by transwell migration assays . Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
resistant
Cancer basic information
Cancer:
lung adenocarcinoma
Tissue/Cell:
cell line (A549, H157,HEK-293T)
Other information
Title:
LncRNA HOXA11-AS drives cisplatin resistance of human LUAD cells via modulating miR-454-3p/Stat3.
Journal:
Cancer Sci
Published:
2018
PubMed ID:
30099826