ncRNA basic information
ncRNA ID:
MI0016786
ncRNA Database:
miRBase
ncRNA Name:
miR-4443
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
INPP4A
ncRNA Pathway:
JAK2/STAT3 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00445 (APRD00361)
Drug Name:
epirubicin
Drug Method:
We aimed to elucidate the roles and regulatory mechanism of miR-4443 in regulating the resistance of non-small cell lung cancer (NSCLC) cells to epirubicin (EPI). Fifty-four advanced NSCLC patients were classified as insensitive or sensitive according to patients responses following EPI-based chemotherapy and then the expression of miR-4443 was determined. The EPI-resistant H1299 cells were collected and transfected with miR-4443 mimics, whereas parental H1299 cells were transfected with miR-4443 inhibitors. The inhibition of growth (IC50), cell cycle or apoptosis of different transfected groups were investigated. Additionally, the potential target of miR-3188 was identified and verified by luciferase reporter assay. Besides, the regulatory relationship between miR-3188 and JAK2/STAT3 pathway was explored.
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line ( H1299)
Other information
Title:
Overexpression of miR-4443 promotes the resistance of non-small cell lung cancer cells to epirubicin by targeting INPP4A and regulating the activation of JAK2/STAT3 pathway.
Journal:
Pharmazie
Published:
2018
PubMed ID:
30001772