ncRNA basic information
ncRNA ID:
MI0000268
ncRNA Database:
miRBase
ncRNA Name:
miR-34a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
TCF1 and LEF1
ncRNA Pathway:
Wnt/Beta-catenin pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00445 (APRD00361)
Drug Name:
epirubicin
Drug Method:
In this study, real-time quantitative polymerase chain reaction (PCR) was used to analyze the expression of miR-34a in bladder cancer cell line BIU87 and its EPI chemoresistant cell line BIU87/ADR. The miR-34a profiles in bladder cancer tissues were obtained from The Cancer Genome Atlas database. The effect of miR-34a on chemosensitivity was evaluated by cell viability assays, colony formation assays, and in vivo experimentation. Apoptosis and the cell cycle were examined by flow cytometry. A luciferase reporter assay was used to assess the target genes of miR-34a. Western blot and qPCR were used to analyze the expression of target proteins and downstream molecules.
Drug Response:
sensitive
Cancer basic information
Cancer:
Bladder Cancer
Tissue/Cell:
cell line (BIU87 and BIU87/ADR )
Other information
Title:
MicroRNA-34a Attenuates Metastasis and Chemoresistance of Bladder Cancer Cells by Targeting the TCF1/LEF1 Axis.
Journal:
Cell Physiol Biochem
Published:
2018
PubMed ID:
30001529