ncRNA basic information
ncRNA ID:
MI0000283
ncRNA Database:
miRBase
ncRNA Name:
miR-203
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
PCR
ncRNA Target Gene:
MEK1
ncRNA Pathway:
ERK/MAPK signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
Dual luciferase assay confirmed the targeted relationship between miR-203 and MEK1. MiR-203, MEK1, p-ERK1/2, and B cell lymphoma 2 (Bcl-2) expressions were compared in normal prostate epithelial cells PrEC, prostate cancer cells PC-3M, and drug resistance cells PC-3M/ADM. PC-3M, PC-3M/ADM cell apoptosis and proliferation were detected by using flow cytometry under ADM treatment at IC50 concentration of PC-3M cells. PC-3M cells were cultured in vitro and divided into four groups, including microRNA-normal control (miR-NC), miR-203 mimic, small interfere NC (si-NC), and si-MEK1.
Drug Response:
sensitive
Cancer basic information
Cancer:
prostate cancer
Tissue/Cell:
cell line ( PC-3M, PC-3M/ADM )
Other information
Title:
MiR-203 over-expression promotes prostate cancer cell apoptosis and reduces ADM resistance.
Journal:
Eur Rev Med Pharmacol Sci
Published:
2018
PubMed ID:
29949147