ncRNA basic information
ncRNA ID:
MI0000076
ncRNA Database:
miRBase
ncRNA Name:
miR-20a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ASK1
ncRNA Pathway:
ROS/ASK1/JNK pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
MTT assays were performed to determine the viability of HT29, SW480, and LoVo cells. Quantitative real time polymerase chain reaction (qRT-PCR) was performed to examine the expression of miR-20a in these cell lines. Regulation of the miR-20a/ASK1 axis was confirmed by western blotting and luciferase reporter assays. After treatment with miR-20a inhibitor (anti-miR-20a) and cisplatin, production of reactive oxygen species (ROS), mitochondrial membrane potential, and apoptosis were measured by flow cytometry. Activation of ASK1, Bcl-xl, JNK, and caspase-9, -7, and -3 was detected by western blotting.
Drug Response:
sensitive
Cancer basic information
Cancer:
colorectal cancer
Tissue/Cell:
cell line ( HT29, SW480)
Other information
Title:
Knockdown of MiR-20a Enhances Sensitivity of Colorectal Cancer Cells to Cisplatin by Increasing ASK1 Expression.
Journal:
Cell Physiol Biochem
Published:
2018
PubMed ID:
29940575