ncRNA basic information
ncRNA ID:
MIMAT0000232
ncRNA Database:
miRBase
ncRNA Name:
miR-199a-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
AK4
ncRNA Pathway:
NF-kappaB pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. We further predicted the putative signal pathway involved in the miR-199a-3p-mediated OS drug-resistance.
Drug Response:
resistant
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (G-292,U2OS, MNNG/HOS)
Other information
Title:
MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4.
Journal:
BMC Cancer
Published:
2018
PubMed ID:
29866054