ncRNA basic information
ncRNA ID:
MI0000285
ncRNA Database:
miRBase
ncRNA Name:
miR-205
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
PTEN
ncRNA Pathway:
PTEN signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs.MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow cytometric analysis of apoptosis.Cell migration was detected by migration and invasion assay.
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
tissue and cell line (A549, SPC-A1, SK-MES-1,16HBE)
Other information
Title:
miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN.
Journal:
Oncol Rep
Published:
2013
PubMed ID:
24084898