ncRNA basic information
ncRNA ID:
MIMAT0004763
ncRNA Database:
miRBase
ncRNA Name:
miR-488-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
PRKDC
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In this study, we demonstrated that miR-488-3p is significantly downregulated in MM clinical specimens and cell lines. Ectopic expression of miR-488-3p resulted in markedly increased drug sensitivity of MM cells in vitro and in vivo. The DNA-activated, catalytic polypeptide (PRKDC), which encodes DNA-dependent protein kinase catalytic subunit (DNA-PKcs), was identified as a direct target of miR-488-3p using luciferase reporter assays, qRT-PCR, and western blotting analyses. PRKDC knockdown by small interfering RNA (siRNA) alone promoted sensitivity of MM cells to cisplatin (DDP) while overexpression of PRKDC partially rescued the miR-488-3p-mediated acceleration of sensitivity to DDP in MM cells.
Drug Response:
sensitive
Cancer basic information
Cancer:
melanoma
Tissue/Cell:
cell line ( A375, B16, SK-MEL-28, WM451,HEMn-LP )
Other information
Title:
MicroRNA-488-3p sensitizes malignant melanoma cells to cisplatin by targeting PRKDC.
Journal:
Cell Biol Int
Published:
2017
PubMed ID:
28328082