ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ABCB1
ncRNA Pathway:
LUCAT1/miR-200c/ABCB1 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00563 (APRD00353)
Drug Name:
Methotrexate
Drug Method:
The expression levels of miRNA were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells .Cell migration was detected by transwell invasion assay.Xenograft in vivo assay was uesd to test the role of LUCAT1 .
Drug Response:
resistant
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (MG-63, U2OS, HOS and SAOS-2, hFOB)
Other information
Title:
Long non-coding RNA LUCAT1 modulates methotrexate resistance in osteosarcoma via miR-200c/ABCB1 axis.
Journal:
Biochem Biophys Res Commun
Published:
2018
PubMed ID:
29170124