ncRNA basic information
ncRNA ID:
MI0000109
ncRNA Database:
miRBase
ncRNA Name:
miR-103
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
COP1
ncRNA Pathway:
PI3K/AKT signal pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
Different concentrations of ADR were used to induce K562 and KASUMI-1 cells, and miR-103 mimic, inhibitor were transfected into K562 and KASUMI-1 cells. Cell viability and proliferation were determined by trypan blue staining and MTT assays for evaluating K562 and KASUMI-1 cells drug resistance. The relationship of miR-103 and COP1, Trib1, and C/EBPalpha were analyzed by qRT-PCR and Western blot. Cell proliferation, viability were detected again. Besides, the expressions of main factors of cell cycle and PI3K/AKT signal pathway were analyzed by Western blot.
Drug Response:
resistant
Cancer basic information
Cancer:
leukemia
Tissue/Cell:
cell line ( K562, KASUMI-1)
Other information
Title:
MicroRNA-103 confers the resistance to long-treatment of adriamycin to human leukemia cells by regulation of COP1.
Journal:
J Cell Biochem
Published:
2018
PubMed ID:
29058777