ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
E-cadherin and PTEN
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00544 (APRD00516, EXPT03204)
Drug Name:
Fluorouracil
Drug Method:
In this study, we examined the effect of inhibition of miR-200c on the sensitivity of HCT-116 cells to 5-FU. HCT-116 cells were transfected with LNA-anti- miR-200c for 48 h. mRNA expression of miR-200c was investigated by qRT-PCR. The protein expression of phosphatase and tensin homolog (PTEN) and E-cadherin were evaluated by western blotting. Annexin V/ PI staining and caspase 3 activity were used to detect apoptosis. LNA-anti-miR-200c inhibited the miR-200c expression in the transfected cells compared with that in the control group. LNA-anti-miR-200c suppressed the expression of PTEN and E-cadherin independent of the presence of the chemotherapeutic drug 5-FU. LNA-anti-miR-200c reduced the 5-FU-induced apoptosis and caspase 3 activity.
Drug Response:
sensitive
Cancer basic information
Cancer:
colorectal cancer
Tissue/Cell:
cell line ( HCT-116 )
Other information
Title:
The Effect of miR-200c Inhibition on Chemosensitivity (5- FluoroUracil) in Colorectal Cancer.
Journal:
Pathol Oncol Res
Published:
2018
PubMed ID:
28411308