ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real Time RT-PCR
ncRNA Target Gene:
E-cadherin, Bcl-2 and Bax
ncRNA Pathway:
PI3K/AKT signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
SGC7901/DDP cells and its parental cell line SGC7901 cells were transfected with miRNA-200c precursor (Pre-200c) and E-cadherin siRNA, respectively. Real-time RT-PCR was used to detect miRNA-200c expression after transfection with Pre-200c in SGC7901/DDP cell line. Drug sensitivities to DDP, 5-fluorouracil (5-FU), paclitaxel, and adriamycin (ADR) after transfection were tested using MTT assay. The proliferation of SGC7901/DDP cells was also detected after transfection. The protein changes of E-cadherin, Bax, and Bcl-2 after transfection were detected by Western blot.
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (SGC7901,SGC7901/DDP )
Other information
Title:
Inhibitory effects of miRNA-200c on chemotherapy-resistance and cell proliferation of gastric cancer SGC7901/DDP cells.
Journal:
Chin J Cancer
Published:
2010
PubMed ID:
21114921