ncRNA basic information
ncRNA ID:
MI0000077
ncRNA Database:
miRBase
ncRNA Name:
miR-21
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
Spry2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00262 (APRD00006)
Drug Name:
Carmustine
Drug Method:
BCNU-sensitive cells (SWOZ2 cells) were transfected with miR-21 agomir and negative control, and BCNU-resistance cells (SWOZ2-BCNU cells) were transfected with miR-21 antagomir and negative control. The Real-time fluorescence quantitative PCR was used to detect and compare the levels of miR-21expression between SWOZ2-BCNU and SWOZ2 cells. The drug sensitivity of these cells to BCNU was determined by Cell Counting Kit-8 (CCK-8) assay. The protein expression of Spry2 was detected by Western blotting.
Drug Response:
resistant
Cancer basic information
Cancer:
glioma
Tissue/Cell:
cell line (SWOZ2,SWOZ2-BCNU)
Other information
Title:
MiR-21 enhanced glioma cells resistance to carmustine via decreasing Spry2 expression.
Journal:
Eur Rev Med Pharmacol Sci
Published:
2017
PubMed ID:
29228450