ncRNA basic information
ncRNA ID:
MI0000542
ncRNA Database:
miRBase
ncRNA Name:
miR-320a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
ADAM10
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The present study performed gain- and loss-of-function analyses by transfecting cells with mimics or inhibitors and subsequently performing colony formation, proliferation and cisplatin-sensitivity assays. Additionally, in vivo xenograft models were also performed. Bioinformatics algorithms, luciferase reporter activity assay and western blotting were used to predict the potential target of miR-320a. Additionally, the effect of knockdown or overexpression of ADAM metallopeptidase domain 10 (ADAM10) on cell growth and chemosensitivity was examined. The expression of miR-320a and ADAM10 was also determined in primary tumors. The present study revealed that the expression of miR-320a was reduced in GC cells and ectopic miR-320a expression significantly inhibited cell growth in vitro and in vivo and enhanced the sensitivity of GC cells to cisplatin. ADAM10 was a direct target of miR-320a in GC. Knockdown of ADAM10 attenuated the proliferative ability of GC cells, and increased the sensitivity of GC cells to cisplatin. The upregulated ADAM10 accelerated cell growth rate and reduced the cisplatin-sensitivity of cells. Clinically, a significantly negative correlation was identified between the expression of miR-320a and mRNA levels of ADAM10 in tumors. The findings of the present study suggested that miR-320a may function as a tumor suppressor in GC progression and potential therapeutic strategies for GC may be based on the miR-320a/ADAM10 axis.
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (SGC-7901, AGS, BGC-823 and MGC-803)
Other information
Title:
miR-320a modulates cell growth and chemosensitivity via regulating ADAM10 in gastric cancer.
Journal:
Mol Med Rep
Published:
2017
PubMed ID:
29152656