ncRNA basic information
ncRNA ID:
MI0000290
ncRNA Database:
miRBase
ncRNA Name:
miR-214
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In this study, we report miR-214 contributes to cisplatin resistance in osteosarcoma cells. Overexpression of miR-214 decreased the cisplatin sensitivity. By establishing an osteosarcoma cisplatin resistant cell line, we find miR-214 is significantly upregulated in cisplatin resistant cells. Moreover, we show miR-214 promotes anaerobic glycolysis rates of osteosarcoma cells but suppresses mitochondrial oxidative phosphorylation. Consistently, cisplatin resistant cells exhibit upregulated glycolysis but decreased mitochondrial oxidative phosphorylation, a phenotype called Warburg effect. Finally, we demonstrate inhibition of glycolysis by either glycolysis inhibitor or miR-214 inhibition significantly re-sensitizes cisplatin resistant osteosarcoma cells.
Drug Response:
resistant
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line
Other information
Title:
miR-214 modulates cisplatin sensitivity of osteosarcoma cells through regulation of anaerobic glycolysis.
Journal:
Cell Mol Biol (Noisy-le-grand)
Published:
2017
PubMed ID:
28980925