ncRNA basic information
ncRNA ID:
MI0003132
ncRNA Database:
miRBase
ncRNA Name:
miR-493
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR,RT-PCR
ncRNA Target Gene:
TCRP1
ncRNA Pathway:
AKT pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Real-time quantitative PCR (qRT-PCR) and In situ hybridization (ISH) were used to analyze the expression of miR-493 in lung cancer cell lines and tumor tissue, respectively. Bisulfite sequencing PCR (BSP) was used to exam the promoter CpG Island of miR-493. The effect of miR-493 on chemosensitivity was evaluated by cell viability assays, apoptosis assays and in vivo experiment. The DNA damage was measured by Gamma-H2AX immunofluorescence. Luciferase reporter assay was used to assess the target genes of miR-493. Expression of target proteins and downstream molecules were analyzed by Western blot.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (H1975, A549, H1299, H460,A549/DDP)
Other information
Title:
Epigenetic silencing of miR-493 increases the resistance to cisplatin in lung cancer by targeting tongue cancer resistance-related protein 1(TCRP1).
Journal:
J Exp Clin Cancer Res
Published:
2017
PubMed ID:
28859669