ncRNA basic information
ncRNA ID:
MI0000100
ncRNA Database:
miRBase
ncRNA Name:
miR-98
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
E2F1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Adriamycin
Drug Method:
Real-time quantitative polymerase chain reaction analyzed the expression difference between miR-98 and E2F1 in leukemia cell lines, K562 and K562/A02. The downstream target gene of miR-98 was predicted by TargetScan; K562/A02 was transiently transfected with miR-98 mimic to upregulate the expression of miR-98; real-time quantitative polymerase chain reaction and Western blot were used to analyze the expression alterations of E2F1; cell counting kit-8 was used to evaluate the influence on K562/A02 proliferation and sensitivity to chemotherapeutic drugs; meanwhile, Western blot was used to analyze the expression of p21, Bax, matrix metalloproteinase 9 and ABCG2 proteins.
Drug Response:
sensitive
Cancer basic information
Cancer:
leukemia
Tissue/Cell:
cell line ( K562,K562/A02 )
Other information
Title:
Targeted regulation of MiR-98 on E2F1 increases chemosensitivity of leukemia cells K562/A02.
Journal:
Onco Targets Ther
Published:
2017
PubMed ID:
28721074