ncRNA basic information
ncRNA ID:
MI0000065
ncRNA Database:
miRBase
ncRNA Name:
let-7d
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The aim of this study includes whether different expression levels of let-7d has an influence on chemo- and radiosensitivity. FaDu cell line models with a gradually increased level of let-7d (models from A to E) were generated with the lentiviral system. Expression levels of pluripotency, chemo-radioresistance/apoptosis, and targets of mRNAs were analyzed by real-time reverse transcription-PCR (qRT-PCR). Radiosensitivity was analyzed using a clonogenic assay after irradiation. Response to cisplatin, 5-FU, doxorubicin, and paclitaxel was done with MTT assay.
Drug Response:
sensitive
Cancer basic information
Cancer:
head and neck squamous cell carcinoma
Tissue/Cell:
cell line ( FaDu )
Other information
Title:
Different levels of let-7d expression modulate response of FaDu cells to irradiation and chemotherapeutics.
Journal:
PLoS One
Published:
2017
PubMed ID:
28665983