ncRNA basic information
ncRNA ID:
MI0000459
ncRNA Database:
miRBase
ncRNA Name:
miR-143
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
CIAPIN1
ncRNA Pathway:
miR-143/CIAPIN1 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Taxol
Drug Method:
The present study aimed to evaluate the therapeutic potential of miR-143 as a treatment for drug-resistant breast cancer via the downregulation of CIAPIN1 in vitro. The expression levels of miR-143 were measured using quantitative polymerase chain reaction and the expression levels of CIAPIN1 were detected via western blot analysis. Bioinformatic analyses was additionally conducted to search for miR-143, which may potentially target CIAPIN1. Luciferase reporter plasmids were created and used to verify direct targeting. In addition, Taxol-induced drug-resistant (TDR) breast cancer cell proliferation was evaluated using the Cell Counting Kit-8 assay in vitro.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7, MDA-MB-231, MDA-MB-453,HEK-293)
Other information
Title:
Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1.
Journal:
Oncol Lett
Published:
2017
PubMed ID:
28588724