ncRNA basic information
ncRNA ID:
MIMAT0000449
ncRNA Database:
miRBase
ncRNA Name:
miR-146a-5p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
NA
ncRNA Target Gene:
XIAP, BCL2L2 and BIRC5
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In this study, the role of miR-146a-5p in cisplatin-induced apoptosis of ovarian cancer cells was assessed by DAPI staining, MTT assays, and monitoring expression of XIAP, BCL2L2, BIRC2 and BIRC5 through a dual-luciferase assay. Our results show that miR-146a-5p can regulate three important anti-apoptotic genes including XIAP, BCL2L2 and BIRC5 via their 3UTRs. Not only can overexpression of miR-146a-5p downregulate the expression of XIAP in SKOV3 cells, but it also lowers the IC50 values of cisplatin in OVCAR3 and SKOV3 cells and enhances the susceptibility of OVCAR3, SKOV3 and primary ovarian cancer cells to cisplatin-induced apoptosis. The effect of XIAP rescuing cisplatin-induced apoptosis accelerated by miR-146a-5p further supports our conclusion. Our results suggest that the regulation of three anti-apoptotic genes by miR-146-5p enhances the therapeutic effects of cisplatin.
Drug Response:
sensitive
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line ( OVCAR3 and SKOV3 )
Other information
Title:
MicroRNA-146a-5p enhances cisplatin-induced apoptosis in ovarian cancer cells by targeting multiple anti-apoptotic genes.
Journal:
Int J Oncol
Published:
2017
PubMed ID:
28560455