ncRNA basic information
ncRNA ID:
MI0000077
ncRNA Database:
miRBase
ncRNA Name:
miR-21
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00853 (APRD00557)
Drug Name:
Temozolomide
Drug Method:
The expression levels of miRNA were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) . Those of protein were by Western blot analysis.Trypan blue dye exclusion test for cell viability. Hoechst 33258 staining for morphological analysis of apoptosis.Caspase-3 activation status was measured using the Caspase-Glo 3 Assay.
Drug Response:
resistant
Cancer basic information
Cancer:
glioblastoma
Tissue/Cell:
cell line ( U87MG)
Other information
Title:
MiR-21 protected human glioblastoma U87MG cells from chemotherapeutic drug temozolomide induced apoptosis by decreasing Bax/Bcl-2 ratio and caspase-3 activity.
Journal:
Brain Res
Published:
2010
PubMed ID:
20633539