ncRNA basic information
ncRNA ID:
MIMAT0000231
ncRNA Database:
miRBase
ncRNA Name:
miR-199a-5p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
Beclin1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (MG63)
Other information
Title:
MicroRNA-199a-5p inhibits cisplatin-induced drug resistance via inhibition of autophagy in osteosarcoma cells.
Journal:
Oncol Lett
Published:
2016
PubMed ID:
27895792