ncRNA basic information
ncRNA ID:
MIMAT0000682
ncRNA Database:
miRBase
ncRNA Name:
miR-200a-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
DUSP6
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00544 (APRD00516, EXPT03204)
Drug Name:
Fluorouracil
Drug Method:
In the study, we investigated whether microRNAs (miRNAs) function as pivotal regulators in the acquisition of anti-cancer drug resistance to 5-fluorouracil (5-FU). A survey using a lentivirus library containing 572 precursor miRNAs revealed that five miRNAs promoted cell survival after 5-FU treatment in human hepatocellular carcinoma Hep3B cells. Among the five different clones, the clone expressing miR-200a-3p (Hep3B-miR-200a-3p) was further characterized as a 5-FU-resistant cell line. The cell viability and growth rate of Hep3B-miR-200a-3p cells were higher than those of control cells after 5-FU treatment. Ectopic expression of a miR-200a-3p mimic increased, while inhibition of miR-200a-3p downregulated, cell viability in response to 5-FU, doxorubicin, and CDDP (cisplatin). We also showed that dual-specificity phosphatase 6 (DUSP6) is a novel target of miR-200a-3p and regulates resistance to 5-FU. Ectopic expression of DUSP6 mitigated the pro-survival effects of miR-200a-3p. Taken together, these results lead us to propose that miR-200a-3p enhances anti-cancer drug resistance by decreasing DUSP6 expression.
Drug Response:
resistant
Cancer basic information
Cancer:
hepatocellular carcinoma
Tissue/Cell:
cell line ( Hep3B-CTRL, Hep3B-miR-200a and Hep3B )
Other information
Title:
microRNA-200a-3p increases 5-fluorouracil resistance by regulating dual specificity phosphatase 6 expression.
Journal:
Exp Mol Med
Published:
2017
PubMed ID:
28496200