ncRNA basic information ncRNA ID: MIMAT0000431 ncRNA Database: miRBase ncRNA Name: miR-140-5p ncRNA Type: miRNA ncRNA Expression: down-regulated ncRNA Method: qRT-PCR ncRNA Target Gene: HMGN5 ncRNA Pathway: NA Evidence (ncRNA-drug): validated

drug basic information Drug ID: DB00997 (APRD00185, DB05331, DB05847) Drug Name: Doxorubicin Drug Method: In this report, we used high-throughput microRNA (miRNA) expression analysis to reveal that expression of miR-140-5p was associated with chemosensitivity in osteosarcoma. The exact roles of miR-140-5p in the chemoresistance of osteosarcoma were then investigated, we found that knockdown of miR-140-5p enhanced osteosarcoma cells resistance to multiple chemotherapeutics while overexpression of miR-140-5p sensitized tumors to chemotherapy in vitro. Moreover, in vivo, knockdown of miR-140-5p also increased the osteosarcoma cells resistance to chemotherapy. Luciferase assay and Western blot analysis showed that HMGN5 was the direct target of miR-140-5p which could positively regulated autophagy. Silencing these target genes by siRNA or inhibition of autophagy sensitized osteosarcoma cells to chemotherapy. These findings suggest that a miR-140-5p/HMGN5/autophagy regulatory loop plays a critical role in chemoresistance in osteosarcoma. In conclusion, our data elucidated that miR-140-5p promoted autophagy mediated by HMGN5 and sensitized osteosarcoma cells to chemotherapy. Drug Response: resistant

Cancer basic information Cancer: osteosarcoma Tissue/Cell: cell line (hFOB1. 19,MG-63, U-2 OS)

Other information Title: MicroRNA-140-5p regulates osteosarcoma chemoresistance by targeting HMGN5 and autophagy. Journal: Sci Rep Published: 2017 PubMed ID: 28341864