ncRNA basic information
ncRNA ID:
MI0000294/MI0000295
ncRNA Database:
miRBase
ncRNA Name:
miR-218
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Glut1
ncRNA Pathway:
miR-218-Glut1 Pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
qRT-PCR was used to detect miR-218 and its target Glut1 expression in bladder cancer cell lines T24 and EJ. CCK-8 method was utilized to measure the cell viability. IC 50 was calculated via a probit regression model. Glut1 was detected by western blotting for analysis of potential mechanism. Luciferase reporter assay was utilized to validate Glut1 as a direct target gene of miR-218. The intracellular level of GSH and ROS were determined using a commercial colorimetric assay kit and 2, 7-dichlorodihydro-fluorescein diacetate, respectively.
Drug Response:
sensitive
Cancer basic information
Cancer:
Bladder Cancer
Tissue/Cell:
cell line ( T24 )
Other information
Title:
MicroRNA-218 Increases the Sensitivity of Bladder Cancer to Cisplatin by Targeting Glut1.
Journal:
Cell Physiol Biochem
Published:
2017
PubMed ID:
28222430