ncRNA basic information
ncRNA ID:
MIMAT0001627
ncRNA Database:
miRBase
ncRNA Name:
miR-433-3p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
CREB
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00853 (APRD00557)
Drug Name:
Temozolomide
Drug Method:
In the study, we investigate the potential role of miR-433 in glioma. Quantitative real-time PCR demonstrated that miR-433-3p and miR-433-5p were low expressed in glioma tissues and cell lines. Functional studies suggested that the overexpression of miR-433-3p suppressed proliferation, induced apoptosis and inhibited invasion and migration of human glioma cells. But the growth and metastasis of glioma cells were not significantly influenced by overexpression of miR-433-5p. In a xenograft model, we also showed that miR-433-3p had an inhibitory effect on the growth of glioma. Bioinformatics coupled with luciferase and western blot assays revealed that CREB is a direct target of miR-433-3p, and the overexpression of CREB can rescue the phenotype changes induced by miR-433-3p overexpression. Besides, miR-433-3p could increase chemosensitivity of glioma to temozolomide by targeting CREB in vitro and in vivo.
Drug Response:
sensitive
Cancer basic information
Cancer:
glioma
Tissue/Cell:
cell line (U251, U87, LN229, SNB19, LN308)
Other information
Title:
MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma.
Journal:
Oncotarget
Published:
2017
PubMed ID:
27926502