ncRNA basic information
ncRNA ID:
MI0000113
ncRNA Database:
miRBase
ncRNA Name:
miR-106a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
ULK1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01254
Drug Name:
Dasatinib
Drug Method:
We screened autophagic activity in A549, H460, and H1299 NSCLC cell lines treated with two different Src-TKIs (saracatinib, dasatinib) or shRNA targeting SRC. The autophagy response was determined by LC3B-I to -II conversion, increased ULK1 epxression and increased GFP-LC3B dot formation. Autophagy was inhibited by pharmacological (bafilomycin A, chloroquine) or genetic (ULK1 shRNA) means. Expression of miR-106a and miR-20b was analyzed by qPCR, and we used different lentivral vectors for ectopic expression of either miR-106a mimetics, anti-sense miR-106a or different miR-106a-363 cluster constructs.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line ( A549, H460, H1299)
Other information
Title:
MicroRNA-106a targets autophagy and enhances sensitivity of lung cancer cells to Src inhibitors.
Journal:
Lung Cancer
Published:
2017
PubMed ID:
27372519