ncRNA basic information
ncRNA ID:
MIMAT0000419
ncRNA Database:
miRBase
ncRNA Name:
miR-27b-3p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NR5A2 and CREB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00675 (APRD00123)
Drug Name:
Tamoxifen
Drug Method:
The expression levels of miRNA were determined by Real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line ( MCF-7,T47D,BT-549, SK-BR-3, MDA-MB-231 and MCF-10A )
Other information
Title:
Downregulation of microRNA-27b-3p enhances tamoxifen resistance in breast cancer by increasing NR5A2 and CREB1 expression.
Journal:
Cell Death Dis
Published:
2016
PubMed ID:
27809310