ncRNA basic information
ncRNA ID:
MIMAT0000250
ncRNA Database:
miRBase
ncRNA Name:
miR-139-5p
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NOTCH1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00541 (APRD00495)
Drug Name:
Vincristine
Drug Method:
The expression levels of miRNA were determined byqRT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells.our in vivo experiments using mouse showed that in CD44+/CD133+ CSC-like cells, miR-139-5p could reverse MDR by downregulating NOTCH1..
Drug Response:
sensitive
Cancer basic information
Cancer:
colorectal carcinoma
Tissue/Cell:
cell line (HCT116, HCT8)
Other information
Title:
MiR-139-5p reverses CD44+/CD133+-associated multidrug resistance by downregulating NOTCH1 in colorectal carcinoma cells.
Journal:
Oncotarget
Published:
2016
PubMed ID:
27738333