ncRNA basic information
ncRNA ID:
MI0000810
ncRNA Database:
miRBase
ncRNA Name:
miR-135b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
FZD1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
To identify the mechanism of miR-135b and FZD1 in NSCLC chemoresistance and to observe their biological functions, we detected the expression levels of miR-135b and FZD1 by conducting quantitative real-time polymerase chain reaction (RT-qPCR) and modified the expressions of miR-135b and FZD1 by transiently transfecting cells with miR-135b mimics or FZD1-siRNA. The 3-untranslated region (3-UTR) of FZD1 combined with miR-135b was verified through dual-luciferase reporter assay.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line (A549,A549/DDP)
Other information
Title:
miR-135b reverses chemoresistance of non-small cell lung cancer cells by downregulation of FZD1.
Journal:
Biomed Pharmacother
Published:
2016
PubMed ID:
27643554