ncRNA basic information
ncRNA ID:
MI0000299
ncRNA Database:
miRBase
ncRNA Name:
miR-222
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00675 (APRD00123)
Drug Name:
Tamoxifen
Drug Method:
The expression levels of miRNA were determined by Reverse Transcription (RT)-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (T47D, BT474, MDA-MB-361, MCF-7, MDA-MB-453, MDA-MB-157, SKBr3, MDA-MB-468, Hs578T, MDA-MB-231, MDA-MB-435s,MCF-10A)
Other information
Title:
MicroRNA-221/222 negatively regulates estrogen receptor alpha and is associated with tamoxifen resistance in breast cancer.
Journal:
J Biol Chem
Published:
2008
PubMed ID:
18790736