ncRNA basic information
ncRNA ID:
MI0000072
ncRNA Database:
miRBase
ncRNA Name:
miR-18a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00675 (APRD00123)
Drug Name:
Tamoxifen
Drug Method:
The expression levels of miRNA were determined by qRT-PCR and those of protein were by Western blot analysis. The putative microRNA (miRNA) binding sites in UCA1 was predicted by using PITA and RNAhybrid . Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells . Soft agar assays were performed to determine the in vitro tumorigenicity of the cells. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line ( MCF-7, BT474, LCC2, and LCC9)
Other information
Title:
Long non-coding RNA UCA1 enhances tamoxifen resistance in breast cancer cells through a miR-18a-HIF1alpha feedback regulatory loop.
Journal:
Tumour Biol
Published:
2016
PubMed ID:
27629141