ncRNA basic information
ncRNA ID:
MIMAT0004811
ncRNA Database:
miRBase
ncRNA Name:
miR-33b-3p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The present study has attempted to identify differentially expressed miRNAs in cisplatin induced DNA damage response in lung cancer cells, and probe into the effects of the misexpressed miRNAs on cisplatin sensitivity. The expression levels of miRNA were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Cell viability used to monitor the growth of the cells
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549,A549/DDP,HEK293T)
Other information
Title:
DNA damage responsive miR-33b-3p promoted lung cancer cells survival and cisplatin resistance by targeting p21WAF1/CIP1.
Journal:
Cell Cycle
Published:
2016
PubMed ID:
27559850