ncRNA basic information
ncRNA ID:
MI0000461
ncRNA Database:
miRBase
ncRNA Name:
miR-145
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
MPR1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Reverse transcription (RT)-polymerase chain reaction (PCR) and real-time PCR . Those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, MCF-10A, MDA-kb2, and MCF-7/ADR)
Other information
Title:
miR-145 sensitizes breast cancer to doxorubicin by targeting multidrug resistance-associated protein-1.
Journal:
Oncotarget
Published:
2016
PubMed ID:
27487127