ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
P53/P21 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00563 (APRD00353)
Drug Name:
Methotrexate
Drug Method:
This study aims to evaluate the function of miR-200c in lung cancer A549 cells. The data presented in our study demonstrated that the expression of miR-200c was down-regulated in methotrexate-resistant A549 cells. Over expression of miR-200c could significantly inhibit cell proliferation, induce G0/G1 cell cycle arrest and induce cell apoptosis. RT-PCR and Western blot assays showed that the expression of P53 and P21 were significantly increased with miR-200c overexpression. These results indicated that over expression of miR-200c might enhance the sensitivity of A549 cells to methotrexate through the P53/P21 pathway. Furthermore, miR-200c overexpression significantly inhibited cell migration and invasion with increasing the expression of E-cad and decreasing the expression of EZH2.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell linen (A549)
Other information
Title:
Over expression of miR-200c suppresses invasion and restores methotrexate sensitivity in lung cancer A549 cells.
Journal:
Gene
Published:
2016
PubMed ID:
27432063