ncRNA basic information
ncRNA ID:
MI0000290
ncRNA Database:
miRBase
ncRNA Name:
miR-214
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
RFWD2
ncRNA Pathway:
p53 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Quantitative real time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF7, MDA-MB-157, MDA-MB-468)
Other information
Title:
miR-214 promotes apoptosis and sensitizes breast cancer cells to doxorubicin by targeting the RFWD2-p53 cascade.
Journal:
Biochem Biophys Res Commun
Published:
2016
PubMed ID:
27422604