ncRNA basic information
ncRNA ID:
MI0003659
ncRNA Database:
miRBase
ncRNA Name:
miR-644a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
CTBP1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Quantitative RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.Cell migration was detected by invasion assay.miRNA target prediction by three databases.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MDA-MB-231, MCF-7, BT474, SK-BR-3, ZR-75-1, MCF-10A, MCF-12A )
Other information
Title:
The miR-644a/CTBP1/p53 axis suppresses drug resistance by simultaneous inhibition of cell survival and epithelial-mesenchymal transition in breast cancer.
Journal:
Oncotarget
Published:
2016
PubMed ID:
27409664