ncRNA basic information
ncRNA ID:
MI0005566
ncRNA Database:
miRBase
ncRNA Name:
miR-374b
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
NA
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
To evaluate the potential contribution of miRNAs in acquired resistance to cisplatin in pancreatic cancer, we compared levels of more than 2000 human miRNAs in a cisplatin-resistant cell line (BxPC3-R) derived from parental (BxPC3) cells by step-wise exposure to increasing concentrations of the drug over more than 20 passages. The acquired drug resistance was accompanied by significant changes in the expression of 57 miRNAs, of which 23 were downregulated and 34 were upregulated. Employing a hidden Markov model (HMM) algorithm, we identified downregulation of miR-374b as likely being directly involved in acquisition of the drug-resistant phenotype. Consistent with this prediction, ectopic overexpression of miR-374b in the resistant BxPC3-R cells restored cisplatin sensitivity to levels approaching those displayed by the BxPC3 parental cells.
Drug Response:
resistant
Cancer basic information
Cancer:
pancreatic cancer
Tissue/Cell:
cell line (BxPC3-R,BxPC3)
Other information
Title:
Evidence for the role of microRNA 374b in acquired cisplatin resistance in pancreatic cancer cells.
Journal:
Cancer Gene Ther
Published:
2016
PubMed ID:
27229158