ncRNA basic information
ncRNA ID:
MI0001446
ncRNA Database:
miRBase
ncRNA Name:
miR-424
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
PD-L1 and CD80
ncRNA Pathway:
PD-L1/PD-1 signalling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by qRT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The production of T-cell cytokines was detected with a cytokine ELISA using the human or mouse ELISA kit.A normalized mRNA expression data set for ovarian cancer was downloaded from the cBioPortal for cancer genomics and used to evaluate PFS and PD-L1, CD80, PD-1, CTLA-4 and miR-424(322) transcript levels.
Drug Response:
sensitive
Cancer basic information
Cancer:
epithelial ovarian carcinoma
Tissue/Cell:
tissue and cell line (OVCAR-3, Skov3 and Skov3)
Other information
Title:
miR-424(322) reverses chemoresistance via T-cell immune response activation by blocking the PD-L1 immune checkpoint.
Journal:
Nat Commun
Published:
2016
PubMed ID:
27147225