ncRNA basic information
ncRNA ID:
MI0003685
ncRNA Database:
miRBase
ncRNA Name:
miR-421
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
E-cadherin and caspase-3
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells. Cell migration was detected by transwell migration and invasion assay.
Drug Response:
resistant
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (AGS, SGC-7901, NCI-N87, HGC-27, HEK293T )
Other information
Title:
MicroRNA-421 regulated by HIF-1alpha promotes metastasis, inhibits apoptosis, and induces cisplatin resistance by targeting E-cadherin and caspase-3 in gastric cancer.
Journal:
Oncotarget
Published:
2016
PubMed ID:
27016414