ncRNA basic information
ncRNA ID:
MI0000446/MI0000470
ncRNA Database:
miRBase
ncRNA Name:
miR-125b
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ErbB2
ncRNA Pathway:
glucose metabolism pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
chondrosarcoma
Tissue/Cell:
tissue and cell line (CSPG, OUMS-27, CH-2879, JJ012, CS-1, SW1353,SNM83)
Other information
Title:
miR-125b acts as a tumor suppressor in chondrosarcoma cells by the sensitization to doxorubicin through direct targeting the ErbB2-regulated glucose metabolism.
Journal:
Drug Des Devel Ther
Published:
2016
PubMed ID:
26966351