ncRNA basic information
ncRNA ID:
MI0001729
ncRNA Database:
miRBase
ncRNA Name:
miR-451a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00675 (APRD00123)
Drug Name:
Tamoxifen
Drug Method:
TAM sensitive cells (MCF-7) and resistant cells (LCC2) were employed in the study. The lentivirus vectors of Lv-miR-451a, Lv-miR-451a sponge, and Lv-miR-451a NC were employed to increase or decrease the expression of miR-451a, respectively. SiRNA to 14-3-3Zeta was used to inhibit expression of 14-3-3Zeta. MTT assay was utilized to detect breast cancer cell proliferation. AnnexinV-FITC binding assay was used to detect apoptosis. Expression of ERalpha, 14-3-3Zeta and miR-451a were measured by qRT-PCR and Western blot analysis. Interactions between 14-3-3Zeta and ERa were investigated by co-immunoprecipitation. LC3-II surface expression and intracellular autophagosomes were observed by Western blot and electron microscopy.
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7,LCC2)
Other information
Title:
Over-expression of miR-451a can enhance the sensitivity of breast cancer cells to tamoxifen by regulating 14-3-3Zeta, estrogen receptor alpha, and autophagy.
Journal:
Life Sci
Published:
2016
PubMed ID:
26896688