ncRNA basic information
ncRNA ID:
MI0000103/MI0000739
ncRNA Database:
miRBase
ncRNA Name:
miR-101
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
Mcl-1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analysis of miR-101 and Mcl-1 mRNA expression.Those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
hepatocellular carcinoma
Tissue/Cell:
cell line (HepG2, Hep3B, Huh7 and PLC human HCC cell lines and the L-O2 normal liver cell line )
Other information
Title:
MicroRNA-101 sensitizes hepatocellular carcinoma cells to doxorubicin-induced apoptosis via targeting Mcl-1.
Journal:
Mol Med Rep
Published:
2016
PubMed ID:
26718267