ncRNA basic information
ncRNA ID:
MI0000434
ncRNA Database:
miRBase
ncRNA Name:
let-7i
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
ABCC10
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by qRT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.Apoptosis assay was performed with Annexin V-FITC/PI Apoptosis Detection Kit .
Drug Response:
sensitive
Cancer basic information
Cancer:
esophageal carcinoma
Tissue/Cell:
cell line (EC109, TE10)
Other information
Title:
BAG3-mediated miRNA let-7g and let-7i inhibit proliferation and enhance apoptosis of human esophageal carcinoma cells by targeting the drug transporter ABCC10.
Journal:
Cancer Lett
Published:
2016
PubMed ID:
26655271